Diego Bonifaz Pérez
Hospital of the National Health Fund, BoliviaTitle: Molecular identification of oncogenic Human Papilloma Virus in genital samples of Bolivian population
Abstract
The present study aims to determine high risk HPV infection in genital samples of the Bolivian population, by applying molecular technology for the genotyping of HPV16 and HPV18 by Real Time PCR. Viral DNA extractions were performed using techniques based on silica columns and the identification of HPV High Risk was through the optimization of a Real Time PCR for the genotyping of HPV16 and 18, for which, the design of primers was carried out and TaqMan® probes by specialized software, and the concentrations of reagents and ideal reaction temperatures were determined, obtaining a duplex assay, useful to determine in the same reaction the HPV16 and 18 genotypes. The highest presence of HPV16 and HPV18 was determined in women aged 15 to 29 years (40% and 26.7% respectively). These results demonstrate that by optimizing and implementing new molecular techniques, a better epidemiological monitoring of the presence of High Risk HPVs can be achieved and thus provide better guidance on the dissemination and presence of the different oncogenic HPV genotypes in our Bolivian population.
Biography
Biochemist by
profession, specialized in Medical Genetics (Universitat of Valencia) and
Tropical Medicine and International Health at the Universitat of Barcelona. He
is currently head of the Molecular Biology Laboratory of the Hospital of the
National Health Fund and Head of the Genetics and Molecular Biology Laboratory
of the "Genetox" Institute in the city of Cochabamba. He also
participates as a teacher in postgraduate courses in Molecular Biology and
Human Genetics at the Autonomous University of Beni and the Private University
of the Valley of Bolivia.
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